골모유사세포주인 MG-63에서 수산화인회석을 이용한 임프란트 표면처리가 세포의 흡착, 성장 및 분화에 미치는 효과
Effect of implant surface microtopography by hydroxyapatite grit-blasting on adhesion, proliferation, and differentiation of osteoblast-like cell line, MG-63
박성재, 배상범, 김수경, 엄태관, 송승일,
소속 상세정보
박성재 ( Park Sung-Jae ) - 아주대학교 의과대학 치과학교실
배상범 ( Bae Sang-Bum ) - 아주대학교 의과대학 치과학교실
김수경 ( Kim Su-Kyoung ) - 임플란트 R&D 센터
엄태관 ( Eom Tae-Gwan ) - 임플란트 R&D 센터
송승일 ( Song Seung-Il ) - 아주대학교 의과대학 치과학교실
KMID : 0355620110370030214
Abstract
Objective: This study examined the potential of the in vitro osteogenesis of microtopographically modified surfaces, RBM (resorbable blasting media) surfaces, which generate hydroxyapatite grit-blasting.
Methods: RBM surfaces were modified hydroxyapatite grit-blasting to produce microtopographically modified surfaces and the surface morphology, roughness or elements were examined. To investigate the potential of the in vitro osteogenesis, the osteoblastic cell adhesion, proliferation, and differentiation were examined using the human osteoblast-like cell line, MG-63 cells. Osteoblastic cell proliferation was examined as a function of time. In addition, osteoblastic cell differentiation was verified using four different methods of an ALP activity assay, a mineralization assay using alizarin red-s staining, and gene expression of osteoblastic differentiation marker using RT-PCR or ELISA.
Results: Osteoblastic cell adhesion, proliferation and ALP activity was elevated on the RBM surfaces compared to the machined group. The cells exhibited a high level of gene expression of the osteoblastic differentiation makers (osteonectin, type Ⅰ collagen, Runx-2, osterix). imilar data was represented in the ELISA produced similar results in that the RBM surface increased the level of osteocalcin, osteopontin, TGF-beta1 and PGE2 secretion, which was known to stimulate the osteogenesis. Moreover, alizarin red-s staining revealed significantly more mineralized nodules on the RBM surfaces than the machined discs.
Conclusion: RBM surfaces modified with hydroxyapatite grit-blasting stimulate the in vitro osteogenesis of MG-63 cells and may accelerate bone formation and increase bone-implant contact.
키워드
Osseointegration; Osteogenesis; Microtopography; Surface roughness; RBM (resorbable blasting media)
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